Sheikh W
Antimicrob Agents Chemother. 1981 Mar;19(3):429-34. doi: 10.1128/AAC.19.3.429.
A neutralizer system was developed and validated for use in the in vitro bactericidal evaluation of three commonly used antiseptics, namely, Hibiclens (4% [wt/vol] chlorhexidine gluconate), Betadine (7.5% [wt/vol] povidone-iodine), and pHisoHex (3% [wt/vol] hexachlorophene). The neutralizer finally selected after a screening of 12 potential candidates consisted of 3% Asolectin, 10% Tween 80, and 0.3% sodium thiosulfate in the recovery agar. This neutralizer system was tested and validated for its neutralizing capacity for the three antiseptics, as well as for its lack of inherent bactericidal action against Staphylococcus aureus and a number of gram-negative bacteria of clinical significance, With no more than a 10-fold dilution of the antiseptic, the selected neutralizer system was 100% effective in neutralizing all the bacteriostatic carry-over of the three antiseptics and was also completely without any inherent bactericidal action against all the test organisms used. Sodium sulfite (considered to be a potential inactivator for iodophores such as Betadine), even in concentrations as low as 0.1%, was found to be ineffective or inherently bactericidal, whereas 0.3% sodium thiosulfate, in combination with Asolectin and Tween 80, was adequate (effective as well as non-bactericidal) and was considered to be essential for the neutralization of the three test antiseptics, namely, Hibiclens, Betadine, and pHisoHex.
开发并验证了一种中和剂系统，用于三种常用防腐剂的体外杀菌评估，这三种防腐剂分别是洗必泰（4%[重量/体积]葡萄糖酸洗必泰）、碘伏（7.5%[重量/体积]聚维酮碘）和pHisoHex（3%[重量/体积]六氯酚）。在对12种潜在候选物进行筛选后最终选定的中和剂由回收琼脂中的3%大豆卵磷脂、10%吐温80和0.3%硫代硫酸钠组成。对该中和剂系统针对这三种防腐剂的中和能力以及对金黄色葡萄球菌和多种具有临床意义的革兰氏阴性菌无固有杀菌作用进行了测试和验证。在防腐剂稀释不超过10倍的情况下，选定的中和剂系统能100%有效中和三种防腐剂的所有抑菌残留，并且对所有使用的测试微生物也完全没有任何固有杀菌作用。亚硫酸钠（被认为是碘伏类如碘伏的潜在灭活剂），即使浓度低至0.1%，也被发现无效或具有固有杀菌作用，而0.3%硫代硫酸钠与大豆卵磷脂和吐温80组合则足够（有效且无杀菌作用），并且被认为是中和三种测试防腐剂（即洗必泰、碘伏和pHisoHex）所必需的。
