Arterial influx of esterified cholesterol from two plasma lipoprotein fractions and its hydrolysis in vivo in hypercholesterolemic rabbits.

Arterial influx of esterified cholesterol from 2 plasma lipoprotein fractions, d less than 1.019 and d greater than 1.019, and influx of plasma free cholesterol were determined in each of 15 hypercholesterolemic rabbits with approximately the same plasma cholesterol concentrations but with different extents of arterial lesions. The procedure consisted of injecting intravenously into recipient rabbits [14C]- or [3H]cholesterol-labeled lipoproteins prepared from donor rabbits. The esterified cholesterol of one lipoprotein fraction was labeled primarily with one isotope and that of the other lipoprotein fraction was labeled with the other isotope. Thoracic aortas were removed 4-6 h after lipoprotein injections. The arterial influx of esterified cholesterol was up to 50 times higher in rabbits with maximal lesions than in those with minimal cholesterol deposits. the arterial influx of cholesteryl ester derived from d less than 1.019 lipoproteins was about equal to that derived from the d greater than 1.019 fraction. The amount of cholesteryl ester in plasma d less than 1.019 was approximately 3 times that in d greater than 1.019. Thus, per unit of cholesteryl ester concentration the d less than 1.019 lipoproteins delivered about 1/3 as much cholesteryl ester to the artery as the lipoproteins in the higher density fractions. some 5-40% of plasma esterified cholesterol which had entered the artery was hydrolyzed in the artery during the experimental period. The influx of free cholesterol that could not be accounted for by the influx of intact plasma lipoproteins was 5-80% of the free cholesterol influx. This excess probably represents free cholesterol influx by an exchange between the plasma lipoproteins and the intimal surface of the artery.