Raaphorst G P, Law P, Kruuv J
Physiol Chem Phys. 1978;10(2):177-91.
The proton spin lattice relaxation time (T1) of mammalian cells subjected to a wide range of salt, sucrose, or DMSO solutions has been measured and minima in the T1 curves for cells treated with all solutes except DMSO were observed. At hypotonic solute concentrations, the increases observed in T1 of the cell sample could be correlated to an increase in the amount of intracellular water. At high solute concentrations, cells shrink losing intracellular water; yet increases in T1 were observed. The increases were attributed to changes in macromolecular conformation resulting in changes in the coordination shells associated with the macromolecules. Since these effects are also observed with sucrose, the basic phenomenon is concluded to be due to osmotic factors. However, ion specific effects were also observed. When protein was dissolved in a wide range of NaCl or KCl solutions, the minimum in the T1 curve was not observed, demonstrating that the effect must be characteristic of the response of intact cells to the various solutions.
已测量了处于各种盐溶液、蔗糖溶液或二甲基亚砜（DMSO）溶液中的哺乳动物细胞的质子自旋晶格弛豫时间（T1），并观察到除DMSO外，用所有溶质处理的细胞的T1曲线都存在最小值。在低渗溶质浓度下，细胞样品T1的增加可能与细胞内水量的增加有关。在高溶质浓度下，细胞收缩，失去细胞内水分；然而，T1仍出现增加。这种增加归因于大分子构象的变化，导致与大分子相关的配位层发生变化。由于蔗糖也会出现这些效应，因此得出基本现象是由渗透因素引起的结论。然而，也观察到了离子特异性效应。当蛋白质溶解在各种氯化钠（NaCl）或氯化钾（KCl）溶液中时，未观察到T1曲线的最小值，这表明该效应必定是完整细胞对各种溶液反应的特征。
