Bernofsky C, Gallagher W J
Biochim Biophys Acta. 1981 May 14;659(1):1-6. doi: 10.1016/0005-2744(81)90264-3.
Mixtures of NADH and NADP+ or NADPH and NAD+ were incubated and periodically assayed for hydrogen transfer by measuring the formation of NADPH and NADH with glutathione reductase (NAD(P)H: oxidized-glutathione oxidoreductase, EC 1.6.4.2) and lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27), respectively. Each mixture showed a steady nonenzymic transfer of hydrogen from the reduced to the oxidized pyridine nucleotide to yield a product that was completely enzymically active. The results demonstrate the specific nonenzymic transfer of hydrogen from NADH and NADPH to the pyridine C-4 position of NADP+ and NAD+, respectively.
