Poletaev A B, Kupriianenko T I
Biokhimiia. 1980 Dec;45(12):2153-7.
A procedure for preparative separation of proteins from bovine brain precipitated in a saturated solution by ammonium sulfate (pH 4.0) and remaining in the supernatant is described. It was shown that not less than 22 fractions (up to 28 fractions considering the "equivocal" reaction) produce different responses to the antiserum against the major protein, i.e. S100.
本文描述了一种从硫酸铵(pH 4.0)饱和溶液沉淀并留存于上清液中的牛脑蛋白质中进行制备性分离的方法。结果表明,不少于22个组分(考虑“不确定”反应则可达28个组分)对针对主要蛋白质即S100的抗血清产生不同反应。