[来自橙色假单胞菌BKMB-548的谷氨酰胺（天冬酰胺）酶]
[Glutamin(asparagin)ase from Pseudomonas aurantiaca BKMB-548].
作者信息
Lebedeva Z I, Berezov T T, Orekhovich V N
出版信息
Biokhimiia. 1981 Jan;46(1):85-91.
PMID:7248377
Abstract
A method for isolation of glutamin (asparagin) ase from Pseudomonas aurantiaca BKMB-548 has been developed. The enzyme preparation is homogeneous during polyacrylamide gel electrophoresis (pH 7.5 and 7.0) with SDS. The pH-optima of the enzyme thermal stability and of the glutaminase activity are equal to 6.0-8.0. At higher pH values the asparaginase activity increases within the pH range of 4-9. The amino acid composition of glutamin(asparagin)ase has been determined.
摘要
已开发出一种从橙色假单胞菌BKMB - 548中分离谷氨酰胺（天冬酰胺）酶的方法。该酶制剂在SDS存在下的聚丙烯酰胺凝胶电泳（pH 7.5和7.0）过程中呈均一状态。该酶热稳定性和谷氨酰胺酶活性的最适pH值为6.0 - 8.0。在较高pH值时，天冬酰胺酶活性在pH 4 - 9范围内增加。已测定了谷氨酰胺（天冬酰胺）酶的氨基酸组成。
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