Fujiwara H, Uemoto M, Tanaka C
Brain Res. 1981 Jul 20;216(2):351-60. doi: 10.1016/0006-8993(81)90137-2.
In vivo release of labeled serotonin ([3H]5-HT) from the parietal cortex was investigated by cortical cup technique and electrical stimulation of midbrain raphe in rats anesthetized with pentobarbital sodium. The spontaneous efflux of tritium from the parietal cortex preload with [3H]5-HT followed a multiphasic exponential course. After 120 min, the rate of efflux appeared to fit the single exponential function (slow phase). Imipramine (10(-6)-10(-3) M) produced a dose-dependent increase in the spontaneous release. When pargyline in concentrations ranging from 10(-4) to 10(-3) M were added to the medium in the cup, the unchanged [3H]5-HT significantly increased in a dose-dependent manner and the slow declining coefficient of tritium efflux significantly decreased in the presence of 10(-4) pargyline. Stimulation of the rostral two-thirds of the dorsal raphe and the lateral 5-HT bundle originating from the dorsal raphe significantly increased the release of [3H]5-HT and its metabolites while stimulation of the caudal one-third of the dorsal raphe did not produce a significant increase in the release of [3H]5-HT and its metabolites. Stimulation of the medium raphe produced no or only a slight increase in the release of [3H]5-HT and its metabolites. These findings are a direct demonstration of the in vivo release of [3H]5-HT from the parietal cortex with stimulation of the dorsal raphe, particularly the rostral two-thirds of the nucleus and provide the neurochemical evidence for the dorsal raphe-cortical 5-HT pathway via the lateral 5-HT bundle.
采用皮质杯技术和电刺激中脑缝际核，对戊巴比妥钠麻醉的大鼠顶叶皮质中标记的5-羟色胺（[3H]5-HT）的体内释放情况进行了研究。预先用[3H]5-HT标记的顶叶皮质中氚的自发流出呈多相指数过程。120分钟后，流出速率似乎符合单指数函数（慢相）。丙咪嗪（10^(-6)-10^(-3)M）使自发释放呈剂量依赖性增加。当向皮质杯中的培养基中加入浓度范围为10^(-4)至10^(-3)M的帕吉林时，未变化的[3H]5-HT以剂量依赖性方式显著增加，并且在存在10^(-4)帕吉林的情况下，氚流出的缓慢下降系数显著降低。刺激背侧缝际核的前三分之二和起源于背侧缝际核的外侧5-羟色胺束，显著增加了[3H]5-HT及其代谢产物的释放，而刺激背侧缝际核的后三分之一并未使[3H]5-HT及其代谢产物的释放显著增加。刺激中缝际核未引起[3H]5-HT及其代谢产物的释放增加或仅引起轻微增加。这些发现直接证明了刺激背侧缝际核，特别是核的前三分之二时，顶叶皮质中[3H]5-HT的体内释放，并为通过外侧5-羟色胺束的背侧缝际核-皮质5-羟色胺通路提供了神经化学证据。
