Fürstenberger G, Richter H, Fusenig N E, Marks F
Cancer Lett. 1981 Jan;11(3):191-8. doi: 10.1016/0304-3835(81)90107-5.
Addition of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) to a murine epidermal cell line leads to an early induction of the arachidonic acid cascade as measured by the release of [14C]arachidonic acid and [14C]prostaglandin E2 into the medium, to an induction of the ornithine decarboxylase and finally to cell proliferation. The results show that TPA induces the same sequence of biochemical events in vitro as in mouse epidermis in vivo. Thus, the in vitro system seems to be suitable to study the role of early membrane effects and membrane lipid turnover in the induction of cell proliferation and hyperplasia by TPA.
将佛波酯12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）添加到小鼠表皮细胞系中，会导致花生四烯酸级联反应的早期诱导，这可通过向培养基中释放[14C]花生四烯酸和[14C]前列腺素E2来衡量，还会诱导鸟氨酸脱羧酶，最终导致细胞增殖。结果表明，TPA在体外诱导的生化事件序列与在体内小鼠表皮中诱导的相同。因此，该体外系统似乎适合研究早期膜效应和膜脂周转在TPA诱导细胞增殖和增生中的作用。
