肿瘤启动子佛波醇12-肉豆蔻酸酯13-乙酸酯在人淋巴细胞中诱导产生一种染色体断裂因子。
Tumor promoter phorbol 12-myristate 13-acetate induces a clastogenic factor in human lymphocytes.
作者信息
Emerit I, Cerutti P A
出版信息
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7509-13. doi: 10.1073/pnas.79.23.7509.
Abstract
The mechanism of the clastogenic action--i.e., the ability to induce chromosomal aberrations--of the tumor promoter phorbol 12-myristate 13-acetate (PMA) was investigated. PMA at 10 and 100 ng/ml induced the formation of a low molecular weight (less than 10,000) clastogenic factor (CF) in phytohemagglutinin-stimulated human blood and lymphocyte cultures. Bovine erythrocyte Cu-Zn superoxide dismutase strongly inhibited PMA clastogenicity, both the formation of CF and the action of previously formed CF. The nonsteroidal anti-inflammatory agents indomethacin, imidazol, and 5,8,11,14-icosatetraynoic acid inhibited PMA clastogenicity and the clastogenic activity of previously formed CF. These results suggest that superoxide radicals and stimulation of the arachidonic acid cascade play a role in PMA-induced clastogenicity and the mechanism of action of the CF. The CF may relate the initial interaction of PMA with the cell membrane to the genome.
摘要
研究了肿瘤促进剂佛波酯12 -肉豆蔻酸酯13 -乙酸酯（PMA）的致断裂作用机制，即诱导染色体畸变的能力。10和100 ng/ml的PMA在植物血凝素刺激的人血和淋巴细胞培养物中诱导形成低分子量（小于10,000）的致断裂因子（CF）。牛红细胞铜锌超氧化物歧化酶强烈抑制PMA的致断裂性，包括CF的形成和先前形成的CF的作用。非甾体抗炎药吲哚美辛、咪唑和5,8,11,14 -二十碳四炔酸抑制PMA的致断裂性和先前形成的CF的致断裂活性。这些结果表明，超氧自由基和花生四烯酸级联反应的激活在PMA诱导的致断裂性和CF的作用机制中起作用。CF可能将PMA与细胞膜的初始相互作用与基因组联系起来。
相似文献
1
Tumor promoter phorbol 12-myristate 13-acetate induces a clastogenic factor in human lymphocytes.肿瘤启动子佛波醇12-肉豆蔻酸酯13-乙酸酯在人淋巴细胞中诱导产生一种染色体断裂因子。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7509-13. doi: 10.1073/pnas.79.23.7509.
2
Clastogenic action of tumor promoter phorbol-12-myristate-13 acetate in mixed human leukocyte cultures.
Carcinogenesis. 1983 Oct;4(10):1313-6. doi: 10.1093/carcin/4.10.1313.
3
Suppression of tumor promoter phorbolmyristate acetate-induced chromosome breakage by antioxidants and inhibitors of arachidonic acid metabolism.抗氧化剂和花生四烯酸代谢抑制剂对肿瘤启动子佛波醇肉豆蔻酸酯乙酸酯诱导的染色体断裂的抑制作用
Mutat Res. 1983 Aug;110(2):327-35. doi: 10.1016/0027-5107(83)90149-5.
4
Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action.肿瘤促进剂佛波醇-12-肉豆蔻酸酯-13-乙酸酯通过间接作用诱导染色体损伤。
Nature. 1981;293(5828):144-6. doi: 10.1038/293144a0.
5
Lipid peroxidation products and clastogenic material in culture media of human leukocytes exposed to the tumor promoter phorbol-myristate-acetate.暴露于肿瘤启动子佛波醇-肉豆蔻酸酯-乙酸酯的人白细胞培养基中的脂质过氧化产物和致断裂物质。
J Free Radic Biol Med. 1985;1(5-6):443-9. doi: 10.1016/0748-5514(85)90159-x.
6
Phorbol ester-induced formation of clastogenic factor from human monocytes.佛波酯诱导人单核细胞产生致断裂因子。
Carcinogenesis. 1987 Apr;8(4):521-6. doi: 10.1093/carcin/8.4.521.
7
Treatment of lymphocyte cultures with a hypoxanthine-xanthine oxidase system induces the formation of transferable clastogenic material.用次黄嘌呤-黄嘌呤氧化酶系统处理淋巴细胞培养物可诱导形成可转移的致染色体断裂物质。
J Free Radic Biol Med. 1985;1(1):51-7. doi: 10.1016/0748-5514(85)90029-7.
8
Effects of epidermal growth factor, phorbol myristate acetate, and arachidonic acid on choriogonadotropin secretion by cultured human choriocarcinoma cells.表皮生长因子、佛波醇肉豆蔻酸酯乙酸盐和花生四烯酸对培养的人绒毛膜癌细胞分泌绒毛膜促性腺激素的影响。
Endocrinology. 1985 Jun;116(6):2400-9. doi: 10.1210/endo-116-6-2400.
9
Induction of human autorosette forming cells by phorbol myristate acetate.佛波醇肉豆蔻酸酯乙酸酯诱导人自身玫瑰花结形成细胞
Clin Exp Immunol. 1982 Sep;49(3):665-9.
10
Hydroxynonenal, a component of clastogenic factors?
Free Radic Biol Med. 1991;10(6):371-7. doi: 10.1016/0891-5849(91)90045-5.
引用本文的文献
1
Optimizing canine T cell activation, expansion, and transduction.优化犬类T细胞的激活、扩增和转导。
PLoS One. 2025 Sep 11;20(9):e0324403. doi: 10.1371/journal.pone.0324403. eCollection 2025.
2
Anti-HIV Activity of Tigliane Derivatives from Roots.来自根部的大戟烷型二萜衍生物的抗HIV活性
Molecules. 2025 Mar 25;30(7):1452. doi: 10.3390/molecules30071452.
3
Smoke treatment triggers the release of a novel DNA damaging factor by lymphocytes.烟雾处理引发淋巴细胞释放一种新型DNA损伤因子。
Mol Cell Biochem. 2006 Mar;284(1-2):73-9. doi: 10.1007/s11010-005-9015-4. Epub 2006 Jan 12.
4
Suppression of development of anti-nuclear antibody and glomerulonephritis in NZB x NZWF1 mice by persistent infection with lactic dehydrogenase virus: possible involvement of superoxide anion as a progressive effector.乳酸脱氢酶病毒持续感染对NZB×NZWF1小鼠抗核抗体产生及肾小球肾炎发展的抑制作用：超氧阴离子作为进展性效应因子的可能参与
Int J Exp Pathol. 1993 Dec;74(6):553-60.
5
Transferable clastogenic activity in plasma from persons exposed as salvage personnel of the Chernobyl reactor.切尔诺贝利反应堆救援人员血浆中的可转移致染色体断裂活性。
J Cancer Res Clin Oncol. 1994;120(9):558-61. doi: 10.1007/BF01221035.
6
Active oxygen acts as a promoter of transformation in mouse embryo C3H/10T1/2/C18 fibroblasts.活性氧在小鼠胚胎C3H/10T1/2/C18成纤维细胞中作为转化促进剂起作用。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2085-7. doi: 10.1073/pnas.81.7.2085.
7
Inhibition of plasmacytoma development in BALB/c mice by indomethacin.吲哚美辛对BALB/c小鼠浆细胞瘤发展的抑制作用。
J Exp Med. 1985 May 1;161(5):996-1012. doi: 10.1084/jem.161.5.996.
8
Lipid peroxide induced DNA damage: protection by turmeric (Curcuma longa).脂质过氧化物诱导的DNA损伤：姜黄（Curcuma longa）的保护作用。
Mol Cell Biochem. 1987 Sep;77(1):3-10. doi: 10.1007/BF00230145.
9
Ataxia-telangiectasia: an inherited disorder of ionizing-radiation sensitivity in man. Progress in the elucidation of the underlying biochemical defect.共济失调毛细血管扩张症：一种人类遗传性电离辐射敏感障碍。潜在生化缺陷阐释的进展。
Hum Genet. 1987 Mar;75(3):197-208. doi: 10.1007/BF00281059.
10
Production of thymine glycols in DNA by radiation and chemical carcinogens as detected by a monoclonal antibody.通过单克隆抗体检测辐射和化学致癌物在DNA中产生的胸腺嘧啶乙二醇。
Br J Cancer Suppl. 1987 Jun;8:113-7.
本文引用的文献
1
Chromosome damaging agent of low molecular weight in the serum of New Zealand black mice.新西兰黑鼠血清中的低分子量染色体损伤剂。
Cytogenet Cell Genet. 1980;26(1):41-8. doi: 10.1159/000131420.
2
Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action.肿瘤促进剂佛波醇-12-肉豆蔻酸酯-13-乙酸酯通过间接作用诱导染色体损伤。
Nature. 1981;293(5828):144-6. doi: 10.1038/293144a0.
3
Arachidonic acid and prostaglandin E2 release and enhanced cell proliferation induced by the phorbol ester TPA in a murine epidermal cell line.佛波酯TPA在小鼠表皮细胞系中诱导花生四烯酸和前列腺素E2释放以及细胞增殖增强。
Cancer Lett. 1981 Jan;11(3):191-8. doi: 10.1016/0304-3835(81)90107-5.
4
Chromosome breakage factor in the plasma of two Bloom's syndrome patients.两名布卢姆综合征患者血浆中的染色体断裂因子。
Hum Genet. 1982;61(1):65-7. doi: 10.1007/BF00291336.
5
Arachidonic acid transformation and tumor production.花生四烯酸转化与肿瘤生成。
Adv Cancer Res. 1981;35:49-79. doi: 10.1016/s0065-230x(08)60908-2.
6
Tumour promoters induce mitotic aneuploidy in yeast.肿瘤启动子可诱导酵母出现有丝分裂非整倍体。
Nature. 1981 Nov 19;294(5838):263-5. doi: 10.1038/294263a0.
7
Tumor promoters and the mechanism of tumor promotion.肿瘤促进剂与肿瘤促进机制。
Adv Cancer Res. 1980;32:1-74. doi: 10.1016/s0065-230x(08)60360-7.
8
9
Tumor promoting agent induces lymphocyte mitogenic factor.肿瘤促进剂诱导淋巴细胞促有丝分裂因子。
Biomedicine. 1981 Mar;35(1):11-3.
10
Specific binding of phorbol ester tumor promoters.佛波酯肿瘤启动子的特异性结合
Proc Natl Acad Sci U S A. 1980 Jan;77(1):567-71. doi: 10.1073/pnas.77.1.567.
Zotero 插件