Gupta P K, Sirover M A
Cancer Res. 1981 Aug;41(8):3133-6.
The cell cycle stimulation of individual species of the uracil DNA glycosylase was examined in WI-38 normal diploid fibroblasts. The nuclear uracil DNA glycosylase was induced as WI-38 cells traversed the cell cycle. In contrast, the specific activity of the mitochondrial glycosylase remained constant during cell proliferation. The two enzyme activities can be further distinguished by their elution patterns on DNA-cellulose, by differential cation sensitivity, and by kinetic differences. The singular stimulation of the nuclear glycosylase in the cell cycle is a further suggestion that normal human cells actively regulate excision repair pathways.
在WI-38正常二倍体成纤维细胞中检测了尿嘧啶DNA糖基化酶各物种的细胞周期刺激情况。随着WI-38细胞经历细胞周期，核尿嘧啶DNA糖基化酶被诱导产生。相比之下，线粒体糖基化酶的比活性在细胞增殖过程中保持恒定。这两种酶活性可通过它们在DNA-纤维素上的洗脱模式、不同的阳离子敏感性以及动力学差异进一步区分。细胞周期中核糖基化酶的单一刺激进一步表明正常人类细胞积极调节切除修复途径。
