在克隆密度下培养成人人类细胞。

Growth of adult human cells in culture at clonal densities.

作者信息

Yasin R, Kundu D, Thompson E J

出版信息

Cell Differ. 1981 May;10(3):131-7. doi: 10.1016/0045-6039(81)90033-6.

Abstract

The effect of varying medium concentrations of fetal calf serum (FCS), horse serum (HS) and chicken embryo extract (EE) on the growth capacity of adult human cells cultured at clonal densities was investigated. The best growth was consistently obtained with medium containing 20 parts FCS and 2 parts heat-treated commercial EE. The growth potential of human muscle cells was not related to the age of the patient from which the biopsy was taken, but was dependent on the microenvironment of the culture and the cell density.

摘要

研究了不同培养基浓度的胎牛血清（FCS）、马血清（HS）和鸡胚提取物（EE）对以克隆密度培养的成人细胞生长能力的影响。始终在含有20份FCS和2份经热处理的商业EE的培养基中获得最佳生长效果。人肌肉细胞的生长潜力与活检所取患者的年龄无关，而是取决于培养的微环境和细胞密度。