Carpentier S, Murawsky M, Carpentier A
Circulation. 1981 Aug;64(2 Pt 2):II90-5.
Numerous cardioplegic solutions have been proposed for myocardial protection during open heart surgery. Their effectiveness has been evaluated by hemodynamic, enzymatic and histologic studies, but the cytotoxicity of these solutions has never been assessed. Human fibroblasts and endothelial cells in tissue culture were incubated for 3 hours at 19 degrees C in 12 of the most commonly used cardioplegic solutions. The solutions were then removed and replaced by tissue culture medium at 37 degrees C. The cytotoxicity of the solution was evaluated by staining the cells with trypan blue after 3 hours and 24 hours. The percentage of dead cells varied from 2--21%. Temperature and addition of blood to the various solutions significantly influenced cytotoxicity. Cardioplegic solutions appear to have different toxicity levels when tested in tissue culture.
人们已经提出了多种心脏停搏液，用于心脏直视手术中的心肌保护。通过血流动力学、酶学和组织学研究对其有效性进行了评估，但这些溶液的细胞毒性从未得到评估。将组织培养中的人成纤维细胞和内皮细胞在19℃下于12种最常用的心脏停搏液中孵育3小时。然后去除这些溶液，并用37℃的组织培养基替换。在3小时和24小时后，通过用台盼蓝对细胞进行染色来评估溶液的细胞毒性。死细胞的百分比在2%至21%之间变化。温度和向各种溶液中添加血液对细胞毒性有显著影响。在组织培养中测试时，心脏停搏液似乎具有不同的毒性水平。
