前列腺素E2和F2α对突触体中3H-去甲肾上腺素积累和释放的影响。

The effects of prostaglandins E2 and F2alpha on synaptosomal accumulation and release of 3H-norepinephrine.

作者信息

Wendel O T, Strandhoy J W

出版信息

Prostaglandins. 1978 Sep;16(3):441-9. doi: 10.1016/0090-6980(78)90223-x.

Abstract

Prostaglandins (PG) of both the E and F series may serve as modulators of norepinephrine (NE) release from peripheral sympathetic neurons. We have studied the effects of PGE2 and PGF2alpha on the accumulation and release of 3H-NE in the CNS using synaptosomes isolated from rat hypothalami. The release of 3H-NE from synaptosomes superfused with Krebs-Ringer bicarbonate buffer was multiphasic with an initial fast release phase followed by a slower release. Raising KCl concentration of the superfusion medium to 56mM during the slow release phase is known to stimulate 3H-NE release. PGE2 (1 X 10(-6)M) attenuated 3H-NE release during the fast phase and reduced the amount of 3H-NE released due to KCl stimulation. At lower concentrations of PGE2 there was no change in the release profile. PGF2alpha was without effect on 3H-NE release at all concentrations tested. The accumulation of 3H-NE was significantly diminished by PGE2 at a concentration of 1 X 10(-6)M, while a lower concentration (1 X 10(-7)M) was ineffective. PGF2alpha had no effect on 3H-NE accumulation at all concentrations investigated.

摘要

E 系列和 F 系列前列腺素（PG）均可作为外周交感神经元去甲肾上腺素（NE）释放的调节剂。我们利用从大鼠下丘脑分离的突触体，研究了前列腺素 E2（PGE2）和前列腺素 F2α（PGF2α）对中枢神经系统中 3H-NE 积累和释放的影响。用 Krebs-Ringer 碳酸氢盐缓冲液灌流的突触体释放 3H-NE 具有多相性，最初为快速释放期，随后释放速度减慢。已知在缓慢释放期将灌流介质的氯化钾浓度提高到 56mM 可刺激 3H-NE 释放。PGE2（1×10⁻⁶M）在快速释放期减弱了 3H-NE 的释放，并减少了因氯化钾刺激而释放的 3H-NE 量。在较低浓度的 PGE2 下，释放曲线没有变化。在所有测试浓度下，PGF2α 对 3H-NE 的释放均无影响。浓度为 1×10⁻⁶M 的 PGE2 显著减少了 3H-NE 的积累，而较低浓度（1×10⁻⁷M）则无效。在所有研究浓度下，PGF2α 对 3H-NE 的积累均无影响。