通过电子捕获气液色谱法分析作为五氟苄酯的A、B、E和F前列腺素。

Analysis of A, B, E, and F prostaglandins as pentafluorobenzyl esters by electron-capture gas-liquid chromatography.

作者信息

Skrinska V A, Butkus A

出版信息

Prostaglandins. 1978 Oct;16(4):571-82. doi: 10.1016/0090-6980(78)90187-9.

Abstract

A new and sensitive method is described for the simultaneous analysis of a mixture containing PGE1, PGE2, PGF1alpha, and PGF2alpha by electron-capture gas-liquid chromatography. During derivatization of the mixture, PGE1 and PGE2 were converted to PGB1 and PGB2, respectively, yielding a mixture of PGB1, PGB2, PGF1alpha, and PGF2alpha trimethylsilyl ether pentafluorobenzyl esters. Gas chromatographic resolution of all four derivatives is sufficient for quantitation of each prostaglandin. The A prostaglandins were analyzed by similar conversion to the respective B prostaglandin derivatives. Minimum detection limits for the B and F prostaglandin derivatives were 10 pg and 1 pg, respectively. Samples of rabbit kidney medulla were incubated and analyzed for A, B, E, and F prostaglandins. The results indicate that the method is capable of high recovery and reproducibility.

摘要

描述了一种通过电子捕获气液色谱法同时分析含有前列腺素E1、前列腺素E2、前列腺素F1α和前列腺素F2α的混合物的新的灵敏方法。在混合物衍生化过程中，前列腺素E1和前列腺素E2分别转化为前列腺素B1和前列腺素B2，得到前列腺素B1、前列腺素B2、前列腺素F1α和前列腺素F2α三甲基硅烷基醚五氟苄酯的混合物。所有四种衍生物的气相色谱分离度足以对每种前列腺素进行定量。通过类似的转化为各自的B类前列腺素衍生物来分析A类前列腺素。B类和F类前列腺素衍生物的最低检测限分别为10皮克和1皮克。对兔肾髓质样品进行孵育，并分析其中的A、B、E和F类前列腺素。结果表明该方法具有高回收率和重现性。