Sundaresan G M, Goehl T J, Prasad V K
J Pharm Sci. 1981 Jun;70(6):702-4. doi: 10.1002/jps.2600700638.
A simple, sensitive, and specific high-performance liquid chromatographic procedure was developed to assay norethindrone--mestranol combination tablets. The method involves a chloroform extraction of a single pulverized tablet. After centrifugation, and aliquot of the supernate was injected into a modular high-performance liquid chromatograph. The effluent from the silica column was monitored serially with a fixed-wavelength UV detector (254 nm) for norethindrone quantitation and a fluorescence detector (230 nm for excitation and 280 nm cutoff filter for emission) for mestranol quantitation. Progesterone was used as an internal standard. The method was employed successfully in content uniformity studies of several brands of commercially available tablets.
开发了一种简单、灵敏且特异的高效液相色谱法来测定炔诺酮-炔雌醇复方片剂。该方法包括对单个研碎片剂进行氯仿萃取。离心后，取上清液的一份注入模块化高效液相色谱仪。用固定波长紫外检测器（254 nm）对硅胶柱流出物进行连续监测以定量炔诺酮，用荧光检测器（激发波长230 nm，发射截止滤光片波长280 nm）定量炔雌醇。使用孕酮作为内标。该方法已成功用于几种市售品牌片剂的含量均匀度研究。
