Higuchi S, Yasui K
Nucleic Acids Symp Ser. 1980(8):s143-5.
The complexes formed between poly-5-bromouridylic acid (poly(BU)) and 2,9-dimethyladenine (m2m9A) or 2-methyladenosine (m2Ado) were investigated. The stoichiometry of the m2m9A-poly(BU) complex was found to be 1:1 as determined by equilibrium dialysis measurements. The CD spectra of the complexes showed the formation of helically ordered structure. This is also confirmed by the sharp melting profiles. A comparison of the CD spectra with that of poly(m2A) . poly(BU) complex revealed a close relationship of the helical structures for these three types of complexes. It was observed that the m2m9A was able to form a thermally more stable complex with the poly(BU) than the m2Ado did.
对聚5-溴尿苷酸（聚（BU））与2,9-二甲基腺嘌呤（m2m9A）或2-甲基腺苷（m2Ado）形成的复合物进行了研究。通过平衡透析测量确定，m2m9A-聚（BU）复合物的化学计量比为1:1。复合物的圆二色光谱表明形成了螺旋有序结构。尖锐的熔解曲线也证实了这一点。将这些复合物的圆二色光谱与聚（m2A）·聚（BU）复合物的光谱进行比较，揭示了这三种复合物螺旋结构之间的密切关系。观察到m2m9A与聚（BU）形成的复合物比m2Ado形成的复合物热稳定性更高。
