Sensitive fluorimetry of adenine, its nucleosides and nucleotides.
作者信息
Yoshioka M, Nakamura A, Iizuka H, Nishidate K, Tamura Z, Miyazaki T
出版信息
Nucleic Acids Symp Ser. 1980(8):s61-3.
PMID:7255198
Abstract
We improved our fluorimetric analysis of adenine compounds by high performance liquid chromatography(HPLC). Bromoacetoaldehyde was better than Chloro- or iodoacetoaldehyde as the reagent of fluorescent derivatization. A moderate reaction for unstable ADP and ATP was found and their fluorescent derivatives were separated by HPLC using Hitachi gel No. 3012-N. The method was applied to determine cAMP in human urines and a catecholamine receptor system. Further, a sensitive fluorescence spectrophotometer was developed. In this micro-HPLC, several ten femtomoles of the adenine compounds were separated.
摘要
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