Hook G, Lai C, Bastacky J, Hayes T
Scan Electron Microsc. 1980(4):27-32.
Evaporative coatings of platinum-carbon and gold on freeze-fractured mouse lung, preserved in frozen-hydrated and freeze-dried states, are studied in secondary emission mode by low temperature scanning electron microscopy. Unfixed, inflated frozen lung is freeze-etched in high vacuum, metal coated and observed frozen-hydrated and freeze-dried, to demonstrate sample shrinkage and coating deformation upon drying the sample. Buckling and cracking of the coatings occur as a result of freeze-drying. Correlative TEM thin film analysis of the coatings is used to estimate film thickness and reticularity. Freeze-drying biological samples is a common SEM preparative technique which causes morphological artifacts due to both freezing and drying. Frozen-hydrated low temperature-SEM investigation can selectively study these artifacts morphologically to distinguish between freezing and drying artifacts.
对保存在冷冻水合状态和冷冻干燥状态下的冷冻断裂小鼠肺上的铂 - 碳和金蒸发涂层，通过低温扫描电子显微镜在二次发射模式下进行研究。未固定、充气的冷冻肺在高真空中进行冷冻蚀刻，进行金属镀膜，并在冷冻水合和冷冻干燥状态下观察，以展示样品干燥时的收缩和涂层变形。冷冻干燥导致涂层出现屈曲和开裂。对涂层进行相关的透射电子显微镜薄膜分析以估计膜厚度和网状结构。冷冻干燥生物样品是一种常见的扫描电子显微镜制备技术，由于冷冻和干燥都会导致形态学假象。冷冻水合低温扫描电子显微镜研究可以选择性地从形态学上研究这些假象，以区分冷冻假象和干燥假象。
