Talstad I, Dalen H, Scheie P, Røli J
Scan Electron Microsc. 1981(Pt 2):319-26.
Scanning electron micrographs of whole blood prepared with cryotechnics demonstrated patterns assumed to be artifacts of the freezing process. A SEM investigation of the effects of freezing and freeze-drying on columns of purified blood proteins has been carried out using quench-freezing and cryo-fracturing followed by freeze-drying. Correlative studies with a light microscope and a transmission electron microscope are included. The resulting patterns depended on the type and concentration of protein as well as on the position within the sample and the orientation of the fracture plane relative to the column axis.
用低温技术制备的全血扫描电子显微镜图像显示出被认为是冷冻过程伪像的图案。利用骤冷冷冻和冷冻断裂，随后进行冷冻干燥，对冷冻和冷冻干燥对纯化血液蛋白柱的影响进行了扫描电子显微镜研究。还包括用光显微镜和透射电子显微镜进行的相关研究。所得图案取决于蛋白质的类型和浓度，以及样品内的位置和断裂平面相对于柱轴的方向。
