虹鳟鱼睾丸中的鱼精蛋白信使核糖核酸在一个非翻译区含有核苷酸序列A-A-U-A-A-A。

Protamine messenger RNA from rainbow trout testis contains the nucleotide sequence A-A-U-A-A-A in an untranslated region.

作者信息

Ferrier L N, Davies P L, Dixon G H

出版信息

Biochim Biophys Acta. 1977 Dec 14;479(4):460-70. doi: 10.1016/0005-2787(77)90039-9.

Abstract

Full-length, complementary DNAs were prepared to rainbow trout protamine mRNA using reverse transcriptase and were labelled during synthesis by the replacement of dATP by [alpha32P]dATP or dTTP by [alpha32P]dTTP. The 32P-labelled protamine complementary DNAs were digested with T4 endonuclease IV. Fragments from the digests were separated in two dimensions, and those discrete fragments which could be identified from both the A-labelled and T-labelled complementary DNAs were subjected to sequence analysis. The sequences described here all arise from the non-coding region. One pentadecanucleotide contained the sequence A-A-U-A-A-A which has been reported by Proudfoot and Brownlee ((1976) Nature 263, 211-214) to occur in the noncoding regions of six other eukaryotic mRNAs.

摘要

使用逆转录酶制备虹鳟鱼鱼精蛋白mRNA的全长互补DNA，并在合成过程中通过用[α32P]dATP替代dATP或用[α32P]dTTP替代dTTP进行标记。用T4核酸内切酶IV消化32P标记的鱼精蛋白互补DNA。消化产物中的片段在二维上进行分离，并且那些可以从A标记和T标记的互补DNA中鉴定出的离散片段进行序列分析。这里描述的序列均来自非编码区。一个十五聚体包含序列A-A-U-A-A-A，Proudfoot和Brownlee（（1976年）《自然》263, 211 - 214）报道该序列存在于其他六种真核mRNA的非编码区。