Ferrier L N, Davies P L, Dixon G H
Biochim Biophys Acta. 1977 Dec 14;479(4):460-70. doi: 10.1016/0005-2787(77)90039-9.
Full-length, complementary DNAs were prepared to rainbow trout protamine mRNA using reverse transcriptase and were labelled during synthesis by the replacement of dATP by [alpha32P]dATP or dTTP by [alpha32P]dTTP. The 32P-labelled protamine complementary DNAs were digested with T4 endonuclease IV. Fragments from the digests were separated in two dimensions, and those discrete fragments which could be identified from both the A-labelled and T-labelled complementary DNAs were subjected to sequence analysis. The sequences described here all arise from the non-coding region. One pentadecanucleotide contained the sequence A-A-U-A-A-A which has been reported by Proudfoot and Brownlee ((1976) Nature 263, 211-214) to occur in the noncoding regions of six other eukaryotic mRNAs.
使用逆转录酶制备虹鳟鱼鱼精蛋白mRNA的全长互补DNA，并在合成过程中通过用[α32P]dATP替代dATP或用[α32P]dTTP替代dTTP进行标记。用T4核酸内切酶IV消化32P标记的鱼精蛋白互补DNA。消化产物中的片段在二维上进行分离，并且那些可以从A标记和T标记的互补DNA中鉴定出的离散片段进行序列分析。这里描述的序列均来自非编码区。一个十五聚体包含序列A-A-U-A-A-A，Proudfoot和Brownlee（（1976年）《自然》263, 211 - 214）报道该序列存在于其他六种真核mRNA的非编码区。
