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[黄地老虎颗粒体病毒(Agrotis segetum (Schiff) )病毒粒子内的DNA包装]

[DNA packing inside viral particles of Agrotis segetum (Schiff) turnip moth granulosis virus].

作者信息

Ulanov B P

出版信息

Vopr Virusol. 1981 Jan-Feb(1):105-10.

PMID:7257315
Abstract

The structure of high molecular (88 million daltons) DNA of turnip moth granulosis virus obtained in the linear and cyclic forms was studied. Electron microscopic examination of the fine structure of DNA isolated from virus particles treated with n-toluene sulphonate-N-cyclohexyl-N'-beta-4 (methyl morpholine)-ethylcarbodiimide were carried out. Modified DNA areas were visualized by two methods: with P32 protein and phosphotungstate acid. In both cases a period of recurrence close to the length of the virus particle was revealed. This study confirmed experimentally the previously suggested model of the existence of structural disorders in DNA caused by bends of superspiral molecule at the ends of the virus particle.

摘要

对以线性和环状形式获得的小菜蛾颗粒体病毒高分子(8800万道尔顿)DNA的结构进行了研究。对用对甲苯磺酸盐-N-环己基-N'-β-4(甲基吗啉)-乙基碳二亚胺处理的病毒颗粒中分离出的DNA精细结构进行了电子显微镜检查。通过两种方法观察到了修饰的DNA区域:用P32蛋白和磷钨酸。在这两种情况下,都发现了一个接近病毒颗粒长度的重复周期。这项研究通过实验证实了先前提出的模型,即病毒颗粒末端超螺旋分子的弯曲导致DNA中存在结构紊乱。

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