Fink B R, Calkins D F
Anesthesiology. 1981 Aug;55(2):172-5. doi: 10.1097/00000542-198108000-00013.
Potassium and glucose are usually lacking in solutions employed for nerve conduction block. The significance of this for impulse conduction was studied in rabbit vagus nerve in vitro, incubated for 2 h Ringer's bicarbonate solution containing or lacking 5 mM glucose and 4 mM potassium chloride (n = 5 for each conditions). The c-fiber actin potential was recorded by periodic stimulation and the Na and K content of the desheathed nerve core was determined at the end of the incubation. In the presence of glucose, apparently normal conduction persisted for at least 2 h, even though the nerves incubated in potassium-free medium lost 20 per cent of their potassium. In the absence of glucose, reversible extinction of conduction was complete in 78 +/- 9 min when external potassium was present, and in 110 +/- 10 min when external potassium was absent. The data suggest that lack of glucose may reinforce C-fiber inexcitability during conduction block and that inclusion of a physiologic amount of potassium chloride in the solution may also be desirable.
用于神经传导阻滞的溶液中通常缺乏钾和葡萄糖。为此，在体外对兔迷走神经进行了研究，将其置于含或不含5 mM葡萄糖和4 mM氯化钾的碳酸氢盐林格溶液中孵育2小时（每种条件下n = 5），以探讨其对冲动传导的意义。通过周期性刺激记录c纤维动作电位，并在孵育结束时测定去鞘神经芯的钠和钾含量。在有葡萄糖存在的情况下，即使在无钾培养基中孵育的神经失去了20%的钾，明显正常的传导仍持续至少2小时。在没有葡萄糖的情况下，当存在外部钾时，传导的可逆性消失在78±9分钟内完成，而当不存在外部钾时，在110±10分钟内完成。数据表明，缺乏葡萄糖可能会在传导阻滞期间增强C纤维的兴奋性，并且在溶液中加入生理量的氯化钾可能也是可取的。
