Sanchez-Ramos J, Diab I M, Wainer B, Dinerstein R J, Roth L J
Stain Technol. 1978 May;53(3):163-8. doi: 10.3109/10520297809111459.
The localization of 3H-opiates in the myenteric plexus of the guinea pig ileum is subject to systematic artifact when stretch preparations of the myenteric plexus are dipped into liquid Kodak NTB-3 emulsion for autoradiography. The cause of the artifact was determined to be a discontinuous distribution, or retraction, of emulsion over plexuses. The apposition of frozen freeze-dried ileal sections to dried photographic emulsion avoids this source of error.
当将豚鼠回肠肌间神经丛的拉伸标本浸入柯达NTB - 3液体乳剂中进行放射自显影时，3H - 阿片类物质在豚鼠回肠肌间神经丛中的定位会出现系统性假象。已确定该假象的原因是乳剂在神经丛上分布不连续或回缩。将冷冻干燥的回肠切片与干燥的照相乳剂贴合可避免这一误差来源。
