Van Blitterswijk W J, Van Hoeven R P, Van der Meer B W
Biochim Biophys Acta. 1981 Jun 22;644(2):323-32. doi: 10.1016/0005-2736(81)90390-4.
This paper presents an interpretation of fluorescence polarization measurements in lipid membranes which are labelled with the apolar probe 1,6-diphenyl-1,3,5-hexatriene. The steady-state fluorescence anisotropy, rs, is resolved into a fast decaying or kinetic component, rf, and an infinitely slow decaying or static component, r infinity. The latter contribution, which predominates in biological membranes, is exclusively determined by the degree of molecular packing (order) in the apolar regions of the membrane; r infinity is proportional to the square of the lipid order parameter. An empirical relation between rs and r infinity is presented, which is in agreement with a prediction based on a theory of rotational dynamics in liquid crystals. This relation enabled us to estimate a lipid structural order parameter directly from simple steady-state fluorescence polarization measurements in a variety of isolated biological membranes. It is shown that major factors determining the order parameter in biomembranes are the temperature, the cholesterol and sphingomyelin content and (in a few systems) the membrane intrinsic proteins.
本文介绍了对用非极性探针1,6 - 二苯基 - 1,3,5 - 己三烯标记的脂质膜进行荧光偏振测量的一种解释。稳态荧光各向异性rs被分解为快速衰减或动力学分量rf和无限缓慢衰减或静态分量r∞。后者在生物膜中占主导，完全由膜非极性区域的分子堆积（有序度）决定；r∞与脂质序参数的平方成正比。给出了rs和r∞之间的经验关系，这与基于液晶旋转动力学理论的预测一致。这种关系使我们能够直接从各种分离的生物膜中的简单稳态荧光偏振测量估计脂质结构序参数。结果表明，决定生物膜序参数的主要因素是温度、胆固醇和鞘磷脂含量以及（在少数系统中）膜内在蛋白。
