膜中脂质和蛋白质的结构顺序：荧光各向异性数据评估
Structural order of lipids and proteins in membranes: evaluation of fluorescence anisotropy data.
作者信息
Jähnig F
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6361-5. doi: 10.1073/pnas.76.12.6361.
Abstract
The limiting long-time value of fluorescence anisotropy in membranes is correlated with the orientational order parameter, which characterizes the structural anisotropy of membranes. Existing experimental results for diphenylhexatriene in lipid bilayers are evaluated for the order parameter of lipid order. Steady-state measurements of fluorescence anisotropy can provide the order parameter in good approximation. Proteins in a fluid lipid phase increase the lipid order parameter so determined. Upon comparison with the order parameter from deuterium magnetic resonance, it is concluded that proteins increase the order of the surrounding lipids in off-normal directions. Order parameters of protein order obtained from the limiting value of protein fluorescence anisotropy are discussed with respect to the influence of lipid order on protein order.
摘要
膜中荧光各向异性的极限长时间值与取向序参量相关，该序参量表征了膜的结构各向异性。针对脂质序的序参量，对脂质双层中六苯基二烯的现有实验结果进行了评估。荧光各向异性的稳态测量能够很好地近似提供序参量。处于流体脂质相中的蛋白质会增加如此测定的脂质序参量。通过与来自氘磁共振的序参量进行比较，得出结论：蛋白质在非正常方向上增加了周围脂质的有序性。从蛋白质荧光各向异性的极限值获得的蛋白质序的序参量，就脂质序对蛋白质序的影响进行了讨论。
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