Seiler N, Bolkenius F N, Knödgen B, Haegele K
Biochim Biophys Acta. 1981 Aug 5;676(1):1-7. doi: 10.1016/0304-4165(81)90002-7.
N1-Acetylspermine has been postulated to be an intermediate in the conversion of spermine to spermidine. This compound, together with N1-acetylspermidine has now been detected in the liver of mice which were pretreated with tetrachloromethane. The following methods were used for the identification of N1-acetylspermine: (a) High-pressure liquid-chromatography of the non-derivatized amines on a reversed-phase column, using octane sulfonate for ion-pairing. (b) Thin-layer chromatography of the dansyl derivatives. (c) Mass spectrometry of the dansyl derivatives. Both chromatographic methods allowed the quantitative estimation of N1-acetylspermine and N1-acetylspermidine in the liver of tetrachloromethane-treated animals.
N1-乙酰精胺被认为是精胺转化为亚精胺过程中的一种中间体。现已在经四氯化碳预处理的小鼠肝脏中检测到该化合物以及N1-乙酰亚精胺。采用以下方法鉴定N1-乙酰精胺：(a) 在反相柱上对未衍生化的胺进行高压液相色谱分析，使用辛烷磺酸盐进行离子配对。(b) 丹磺酰衍生物的薄层色谱分析。(c) 丹磺酰衍生物的质谱分析。两种色谱方法均可对四氯化碳处理动物肝脏中的N1-乙酰精胺和N1-乙酰亚精胺进行定量测定。
