Kiziriia E L, Mdzinarashvili T D
Biofizika. 1981 Mar-Apr;26(2):216-21.
Viscosity of DNA solutions of phage T2 at different rate gradients was investigated in relation to ionic strength and solution temperature. Measurements were performed by means of rotation viscosimeter with a magnetic support of the rotor. The slope of gradient relationship was shown to increase with a decrease of ionic strength of DNA solution. A significant temperature relationship of solution viscosity at T less than Tm was observed. A slope of the curves eta sp = f (T) depends on DNA concentration in solution. It was shown that in the presence of Cu+2 or Mg+2 ions at a cyclic change of temperature a small irreversible change of DNA solution viscosity was observed.
研究了噬菌体T2的DNA溶液在不同速率梯度下的粘度与离子强度和溶液温度的关系。测量是通过带有转子磁性支撑的旋转粘度计进行的。结果表明,梯度关系的斜率随DNA溶液离子强度的降低而增加。在T小于Tm时观察到溶液粘度与温度有显著关系。曲线ηsp = f (T)的斜率取决于溶液中DNA的浓度。结果表明,在Cu+2或Mg+2离子存在的情况下,温度循环变化时,DNA溶液粘度会发生小的不可逆变化。