跨紧密上皮的钠离子转运速率的函数关系的细胞内钠离子活性
Intracellular Na+ activity as a function of Na+ transport rate across a tight epithelium.
作者信息
Wills N K, Lewis S A
出版信息
Biophys J. 1980 Apr;30(1):181-6. doi: 10.1016/S0006-3495(80)85086-7.
Abstract
Liquid Na+ resin microelectrodes were used to measure intracellular Na+ activities (alpha iNa+) in the mammalian tight epithelium, rabbit urinary bladder. alpha iNa+ averaged 7 +/- 1 mM and was independent of Na+ transport rate over the range of 2 to 8 muA/muF. (1 mF congruent to 1 cm2 apical membrane area). After Na+ loading the cells the Na+ pump in the basolateral membrane was measurably electrogenic. A method for shielding the Na+-sensitive microelectrodes is described which increases the response time and eliminates an electrical shunting artifact.
摘要
使用液态钠离子树脂微电极来测量哺乳动物紧密上皮组织（兔膀胱）中的细胞内钠离子活性（αiNa+）。αiNa+的平均值为7±1 mM，并且在2至8 μA/μF的范围内与钠离子转运速率无关。（1 mF相当于1 cm2顶膜面积）。在细胞加载钠离子后，可测量到基底外侧膜中的钠泵具有电生性。本文描述了一种用于屏蔽钠离子敏感微电极的方法，该方法可增加响应时间并消除电分流伪影。
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本文引用的文献
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The mechanism of Na+ transport by rabbit urinary bladder.兔膀胱对钠离子的转运机制。
J Membr Biol. 1976 Aug 27;28(1):41-70. doi: 10.1007/BF01869690.
2
Basolateral membrane potential of a tight epithelium: ionic diffusion and electrogenic pumps.紧密上皮细胞的基底外侧膜电位：离子扩散与电生泵
J Membr Biol. 1978 Jun 28;41(2):117-48. doi: 10.1007/BF01972629.
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Sodium-selective liquid ion-exchanger microelectrodes for intracellular measurements.
Science. 1979 Mar 30;203(4387):1349-51. doi: 10.1126/science.424756.
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Na+ transport by rabbit urinary bladder, a tight epithelium.兔膀胱（一种紧密上皮组织）对钠离子的转运。
J Membr Biol. 1976 Aug 27;28(1):1-40. doi: 10.1007/BF01869689.
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