钙离子与肌钙蛋白C结合的激光拉曼光谱研究。
A laser Raman spectroscopic study of Ca2+ binding to troponin C.
作者信息
Carew E B, Leavis P C, Stanley H E, Gergely J
出版信息
Biophys J. 1980 May;30(2):351-8. doi: 10.1016/S0006-3495(80)85099-5.
Abstract
Laser Raman spectroscopy has been used detect structural changes in troponin C induced by Ca2+ binding. Addition of Ca2+ - Mg2+ sites produces perturbations in the amide III region of the spectrum indicative of increased alpha-helical content, and in regions of the spectrum corresponding to carboxylate, thiol, and phenol side chains. However, Ca2+ binding to the low affinity Ca2+ - specific sites is not detected by laser Raman spectral changes.
摘要
激光拉曼光谱已被用于检测由钙离子结合诱导的肌钙蛋白C的结构变化。添加钙离子-镁离子位点会在光谱的酰胺III区域产生扰动，这表明α-螺旋含量增加，并且在光谱中对应于羧酸盐、硫醇和酚侧链的区域也会产生扰动。然而，激光拉曼光谱变化未检测到钙离子与低亲和力钙离子特异性位点的结合。
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