Kukita F, Yamagishi S
Biophys J. 1981 Jul;35(1):243-7. doi: 10.1016/S0006-3495(81)84785-6.
The excitation of the squid giant axon that had been perfused intra- and extracellularly with solutions containing a high concentration of glycerol could be observed below 0 degree C. The action potential could be elicited at normal strengths of electrical stimuli. The time-course of the action potential was slowed, whereas the resting potential and the amplitude of the action potential changed only slightly. The membrane current under the voltage clamp at -6.3 degrees C was about 100-fold slower than that in normal sea water at 8.7 degrees C because of the large viscosity of glycerol solutions and the low temperature. The Q10 values of the magnitude and the time-course of the membrane current were 2.3 and 1/4.0, respectively.
用含有高浓度甘油的溶液进行细胞内和细胞外灌注的枪乌贼巨轴突，在0摄氏度以下仍可观察到兴奋现象。在正常强度的电刺激下能够引发动作电位。动作电位的时程变慢，而静息电位和动作电位的幅度仅略有变化。由于甘油溶液的高粘度和低温，在-6.3摄氏度电压钳制下的膜电流比在8.7摄氏度的正常海水中慢约100倍。膜电流大小和时程的Q10值分别为2.3和1/4.0。
