Genest D, Sabeur G, Wahl P, Auchet J C
Biophys Chem. 1981 Feb;13(1):77-87. doi: 10.1016/0301-4622(81)80027-0.
The fluorescence anisotropy decays of the chromatin ethidium complexes have been measured in solutions in which the dye was bound to the high affinity sites of the nucleosome DNA. Energy transfers between chromatin-bound ethidium molecules cause an increase of naked DNA-ethidium complexes. This result implies that the high affinity sites are clustered on a short nucleosomal DNA segment. Quantitative analysis of the experimental data by computer simulations of the energy transfer process, shows that these sites are gathered on a single nucleosomal DNA segment, 28 base pairs long. Such a segment probably belongs to the nucleosomal "linker", contributing about half of it.
已在染料与核小体DNA高亲和力位点结合的溶液中测量了染色质溴化乙锭复合物的荧光各向异性衰减。染色质结合的溴化乙锭分子之间的能量转移导致裸露的DNA-溴化乙锭复合物增加。这一结果表明，高亲和力位点聚集在短的核小体DNA片段上。通过对能量转移过程进行计算机模拟对实验数据进行定量分析表明，这些位点聚集在一个28个碱基对长的单核小体DNA片段上。这样一个片段可能属于核小体“连接区”，约占其一半。
