Hassinen I E, Hiltunen J K, Takala T E
Cardiovasc Res. 1981 Feb;15(2):86-91. doi: 10.1093/cvr/15.2.86.
Reflectance spectrophotometry was assessed as a means of detecting metabolic oxidation-reduction changes in cytochromes and the oxygenation level of myoglobin in isolated perfused rat hearts. The movement artefact due to the heartbeat was largely eliminated by appropriate design of the optical geometry. A considerable myocardial oxygen gradient was detected using myoglobin deoxygenation as an indicator. The oxygenation level of myoglobin oscillated in pace with the contraction/relaxation cycle of the heart, deoxygenation occurring during the systole. The inconsistency in the previous reports on the myoglobin oxygenation and the problems of spectrophotometry of the beating heart are discussed.
反射分光光度法被评估为一种检测离体灌注大鼠心脏中细胞色素代谢氧化还原变化及肌红蛋白氧合水平的方法。通过适当设计光学几何结构，很大程度上消除了由心跳引起的运动伪影。以肌红蛋白脱氧作为指标，检测到了相当大的心肌氧梯度。肌红蛋白的氧合水平随心脏的收缩/舒张周期同步振荡，在收缩期发生脱氧。讨论了先前关于肌红蛋白氧合的报道中的不一致之处以及跳动心脏分光光度法的问题。
