Cardiac Muscle Research Laboratory, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Am J Physiol Heart Circ Physiol. 2012 Jul 15;303(2):H156-67. doi: 10.1152/ajpheart.00333.2012. Epub 2012 May 25.
The isolated retrograde-perfused Langendorff heart and the isolated ejecting heart have, over many decades, resulted in fundamental discoveries that form the underpinnings of our current understanding of the biology and physiology of the heart. These two experimental methodologies have proven invaluable in studying pharmacological effects on myocardial function, metabolism, and vascular reactivity and in the investigation of clinically relevant disease states such as ischemia-reperfusion injury, diabetes, obesity, and heart failure. With the advent of the genomics era, the isolated mouse heart preparation has gained prominence as an ex vivo research tool for investigators studying the impact of gene modification in the intact heart. This review summarizes the historical development of the isolated heart and provides a practical guide for the establishment of the Langendorff and ejecting heart preparations with a particular emphasis on the murine heart. In addition, current applications and novel methods of recording cardiovascular parameters in the isolated heart preparation will be discussed. With continued advances in methodological recordings, the isolated mouse heart preparation will remain physiologically relevant for the foreseeable future, serving as an integral bridge between in vitro assays and in vivo approaches.
在过去的几十年里，离体逆行灌流Langendorff 心脏和离体射血心脏的研究取得了许多基础性发现，这些发现构成了我们目前对心脏生物学和生理学的理解基础。这两种实验方法在研究心肌功能、代谢和血管反应的药理学效应以及研究缺血再灌注损伤、糖尿病、肥胖和心力衰竭等临床相关疾病状态方面非常有价值。随着基因组学时代的到来，离体小鼠心脏模型作为一种研究基因修饰对完整心脏影响的体外研究工具，受到了越来越多的关注。本文综述了离体心脏的历史发展，并提供了 Langendorff 和射血心脏模型建立的实用指南，特别强调了小鼠心脏模型。此外，还将讨论离体心脏模型中心血管参数记录的当前应用和新方法。随着方法学记录的不断进步，在可预见的未来，离体小鼠心脏模型仍将具有生理相关性，成为体外检测和体内研究之间不可或缺的桥梁。
