Cowall D E, MacVittie T J, Parker G A, Weinberg S R
Exp Hematol. 1981 Jul;9(6):581-7.
Low-dose total-body irradiation, 150 rad given in 10 fractions over 5 weeks, is a useful treatment modality for favorable-prognosis lymphomas. Little is known, however, about the effects of this regimen on normal bone marrow. Six healthy beagle dogs and 5 dogs of various breeds with lymphoma were treated with total-body irradiation. Three of the 5 lymphomatous dogs achieved remissions of limited duration. No changes in hemograms or in bone marrow cellularity (as assessed by needle marrow biopsies) could be detected during or after treatment. Bone marrow progenitor cells were studied weekly during treatment and for 4 weeks thereafter using in vitro growth assays for GM-CFC and M-CFC. These studies demonstrated significant reductions (P less than 0.001) of granulocyte and macrophage progenitor cells with subsequent recovery toward normal pre-irradiation and sham irradiation values. Two additional dogs were injected with sublethal doses of Salmonella typhosa endotoxin 2 weeks after completion of the irradiation regimen. Their bone marrow GM-CFC responses were dramatically blunted compared to nonirradiated controls whereas their peripheral leukocyte responses and serum CSF levels were comparable to nonirradiated controls. These studies suggest that total-body irradiation may induce bone marrow injury that may be clinically significant if patients so treated are further stressed by infections or myelosuppressive drugs.
低剂量全身照射，5周内分10次给予150拉德，是治疗预后良好型淋巴瘤的一种有效治疗方式。然而，对于该方案对正常骨髓的影响却知之甚少。对6只健康的比格犬和5只患有淋巴瘤的不同品种犬进行了全身照射治疗。5只患淋巴瘤的犬中有3只实现了有限期的缓解。在治疗期间或治疗后，未检测到血常规或骨髓细胞密度（通过针吸骨髓活检评估）有变化。在治疗期间每周以及此后4周，使用粒细胞-巨噬细胞集落形成细胞（GM-CFC）和巨噬细胞集落形成细胞（M-CFC）的体外生长试验对骨髓祖细胞进行研究。这些研究表明，粒细胞和巨噬细胞祖细胞显著减少（P小于0.001），随后恢复至照射前和假照射时的正常水平。在照射方案完成2周后，给另外2只犬注射了亚致死剂量的伤寒沙门氏菌内毒素。与未照射的对照组相比，它们的骨髓GM-CFC反应明显减弱，而它们的外周白细胞反应和血清集落刺激因子（CSF）水平与未照射的对照组相当。这些研究表明，全身照射可能会导致骨髓损伤，如果接受这种治疗的患者因感染或骨髓抑制药物而进一步受到压力，这种损伤可能具有临床意义。
