从人血清中纯化一种核酸酶。
Purification of a nuclease from human serum.
作者信息
Zöllner E J, Seibert G, Slor H, Zahn R K
出版信息
Experientia. 1981 Jun;37(6):548-50. doi: 10.1007/BF01990041.
PMID:7262274
Abstract
The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.
摘要
本文描述了从人血清中纯化一种核酸酶的过程。该过程包括硫酸铵沉淀、在DEAE-葡聚糖凝胶和Sephacryl-S 200上进行色谱分离以及制备性电泳。纯化后的酶约为原来的2000倍，在十二烷基硫酸钠电泳系统中呈均一状态，其分子量为78,000。最适pH约为6.5；它是一种对糖不具有特异性的内切核酸酶。
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