Basset G, Martet G, Bouchonnet F, Marsac J, Sutton J, Botter F, Capitini R
J Appl Physiol Respir Environ Exerc Physiol. 1981 Jun;50(6):1367-71. doi: 10.1152/jappl.1981.50.6.1367.
A spectrophotometer designed to measure simultaneously traces of deuterium oxide (12 mg.1-) and indocyanine green (0.12 mg.1-1) in flowing blood is described. Its symmetrical four-beam design enabled direct readings in optical density units to be obtained in the infrared region without interference due to atmospheric CO2 and water. This system was validated by the measures of pulmonary extravascular water in the rat in heart-lung preparations and in whole animals. An example of its application showed that, with this system, it was possible to evaluate correctly an alloxan edema in the rat a short time (105 min) after its induction. In these experiments lung water calculated by indicator curves represented 80% of the lung water content (r = 085, n = 10).
本文描述了一种用于同时测量流动血液中痕量氧化氘（12毫克/升）和吲哚菁绿（0.12毫克/升）的分光光度计。其对称的四光束设计能够在红外区域直接获得以光密度单位表示的读数，而不受大气中二氧化碳和水的干扰。该系统通过在心肺制备的大鼠和完整动物中测量肺血管外水得到了验证。其应用实例表明，使用该系统能够在大鼠诱导四氧嘧啶水肿后短时间内（105分钟）正确评估水肿情况。在这些实验中，通过指示剂曲线计算得到的肺水占肺水含量的80%（r = 0.85，n = 10）。
