Kennedy J F, Barnes J A
J Chromatogr. 1981 Jul 31;212(2):179-86. doi: 10.1016/s0021-9673(00)83053-8.
Class specific F(ab')2 antibody fragments were prepared by pepsin digestion of the labile Fc immunoglobulin fragments in antigen--antibody precipitates. The F(ab')2 fragments were covalently coupled to cyanogen bromide-activated Sepharose 4B and the resultant immunoadsorbent used to isolate IgG from human serum with a single chromatographic step, in high yield and purity and negligible non-specific interaction. This technique affords a simple method for preparing an enriched source of class specific affinity-purified immunoglobulin antibodies suitable for many immunochemical applications.
通过胃蛋白酶消化抗原 - 抗体沉淀物中不稳定的Fc免疫球蛋白片段来制备类特异性F(ab')2抗体片段。将F(ab')2片段与溴化氰活化的Sepharose 4B共价偶联，所得免疫吸附剂用于通过一步色谱法从人血清中分离IgG，产率高、纯度高且非特异性相互作用可忽略不计。该技术提供了一种简单的方法，用于制备适合许多免疫化学应用的类特异性亲和纯化免疫球蛋白抗体的富集来源。
