Tung K S, Teuscher C, Goldberg E H, Wild G
J Immunol. 1981 Sep;127(3):835-9.
About 50% of vasectomized and none of sham-operated outbred Hartley GP develop autoantibodies to TSDA, surface antigens on sperm, spermatids, and residual bodies detectable by a sensitive quantitative cellular radioimmunobinding assay. A similar study of anti-TSDA antibody responses on different strains of GP demonstrates that Strain 13 GP are high responders and PLLhr guinea pigs are nonresponders, whereas the responses of Strain 2 and PLLlr GP are intermediate. Furthermore, 100% of vasectomized F1 GP between Strain 13 females and PLLhr males produce anti-TSDA antibody. Of the vasectomized back-cross GP between the F1 and PLLhr, 46% produce anti-TSDA antibody, 54% do not. These data provide strong evidence that autoimmune antibody response to sperm surface antigens in vasectomized GP is controlled by a single dominant autosomal or X-linked gene. Antisera from vasectomized Strain 13, F1, and back-cross GP are found to react with 3 major polypeptides (MW 63, 37, and 22 kd) on sperm surface that can be radioiodinated and extracted by NP-40.
在接受输精管切除术的远交系Hartley豚鼠中，约50%会产生针对精子表面、精子细胞、残余小体上的睾丸特异性酸性糖蛋白（TSDA）的自身抗体，而假手术组则无一产生，这可通过灵敏的细胞定量放射免疫结合试验检测到。一项关于不同品系豚鼠抗TSDA抗体反应的类似研究表明，13品系豚鼠是高反应者，PLLhr豚鼠无反应，而2品系和PLLlr豚鼠的反应介于两者之间。此外，13品系雌性和PLLhr雄性杂交的F1代豚鼠在输精管切除术后，100%产生抗TSDA抗体。在F1代与PLLhr回交的输精管切除豚鼠中，46%产生抗TSDA抗体，54%不产生。这些数据有力地证明，输精管切除豚鼠对精子表面抗原的自身免疫抗体反应受一个显性常染色体或X连锁基因控制。已发现输精管切除的13品系、F1代和回交豚鼠的抗血清与精子表面3种主要多肽（分子量63、37和22kd）发生反应，这些多肽可进行放射性碘化并能用NP - 40提取。
