Kawai Y, Suegara N, Yazawa K
Mech Ageing Dev. 1981 Jun;16(2):149-58. doi: 10.1016/0047-6374(81)90091-9.
The influence of intestinal microflora and aging on the lipid metabolism in germ-free (GF) and conventional (CV) rats, 8 and 40 weeks old, was investigated. Serum cholesterol at the age of 8 and 40 weeks and serum triglyceride (TG) at the age of 40 weeks was higher in GF than in CV rats. Serum cholesterol decreased and serum TG and corticosterone tended to increase in both GF and CV rats with aging. In the rats 40 weeks of age, lipase activity of the pancreas and the duodenal, jejunal, and colorectal contents in GF rats increased, but that of the ileal and cecal contents in GF and CV rats decreased. Intestinal microflora tended to depress the age-related increase of serum TG and lipase activity of the pancreas and the duodenal and jejunal contents. Lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) activities of the epididymal adipose tissue were higher in CV than in GF rats at both 8 to 40 weeks of age. The LPL activity increased and the HSL activity decreased in both GF and CV rats with aging. The concentration of cholesterol increased and that of bile acids decreased in the cecal contents of 40-week-old GF rats.
研究了肠道微生物群和衰老对8周龄和40周龄无菌（GF）大鼠和普通（CV）大鼠脂质代谢的影响。8周龄和40周龄时，GF大鼠的血清胆固醇以及40周龄时的血清甘油三酯（TG）均高于CV大鼠。随着年龄增长，GF和CV大鼠的血清胆固醇均降低，血清TG和皮质酮均有升高趋势。在40周龄的大鼠中，GF大鼠胰腺以及十二指肠、空肠和结肠内容物的脂肪酶活性增加，但GF和CV大鼠回肠和盲肠内容物的脂肪酶活性降低。肠道微生物群倾向于抑制血清TG以及胰腺、十二指肠和空肠内容物脂肪酶活性随年龄的增加。在8至40周龄期间，CV大鼠附睾脂肪组织的脂蛋白脂肪酶（LPL）和激素敏感性脂肪酶（HSL）活性均高于GF大鼠。随着年龄增长，GF和CV大鼠的LPL活性均增加，HSL活性均降低。40周龄GF大鼠盲肠内容物中胆固醇浓度升高，胆汁酸浓度降低。
