Nashed N
Mutat Res. 1981 Aug;85(4):207-14. doi: 10.1016/0165-1161(81)90037-6.
A new carcinogenicity test system in rats, in vivo-in vitro, is discussed in the light of the validation results obtained so far. The new test lasts only 3 weeks and it involves the use of peritoneal cells as target cells. Colony growth in soft agar is considered as a criterion for cell transformation which, in turn, is taken as evidence for a carcinogenic potential of the agent tested. There was an absence of colony growth in 98% of the cultures made from the 225 control rats tested so far. Negative results were also observed with 2 known non-carcinogens, whereas positive cultures were observed for 7 known carcinogens belonging to 5 different classes. These results suggest that the chosen target cells are sensitive enough to detect the carcinogenic effect of agents applied in vivo even if these are strictly organotropic in their action. Several interesting areas of future research are discussed.
根据目前获得的验证结果，对一种新的大鼠体内-体外致癌性测试系统进行了讨论。新测试仅持续3周，涉及使用腹膜细胞作为靶细胞。软琼脂中的集落生长被视为细胞转化的标准，而细胞转化又被视为受试物致癌潜力的证据。在迄今为止测试的225只对照大鼠所制备的培养物中，98%没有集落生长。对于2种已知的非致癌物也观察到阴性结果，而对于属于5个不同类别的7种已知致癌物则观察到阳性培养物。这些结果表明，即使受试物的作用具有严格的器官特异性，所选择的靶细胞也足够敏感，能够检测到体内应用受试物的致癌作用。文中还讨论了未来研究的几个有趣领域。
