Nielsen J B, Andersen O
Mutat Res. 1986 Feb;173(2):153-6. doi: 10.1016/0165-7992(86)90094-1.
The peritoneal cell carcinogenicity test, which is a new short-term in vivo-in vitro transformation test invented by Nashed (1981), was evaluated in the present report. The experimental design and materials used were as close as possible to those used by Nashed (1981). Test compounds were the two carcinogenic/non-carcinogenic analog pairs benzo[a]pyrene/pyrene and 2-acetylamidofluorene/4-acetylamidofluorene. All 4 compounds were administered orally. Al(OH)3 was injected intraperitoneally to act as mitogen for peritoneal macrophages. The criterium for transformation was colony growth of macrophages in soft agar. Two transformed cell types formed colonies under the conditions used. Pulmonary and peritoneal macrophages formed colonies when colony-stimulating factor was added. No colony growth was observed on plates with macrophages from 256 animals tested (100 carcinogen treated, 80 treated with noncarcinogenic analogs and 76 untreated controls). Thus we were not able to confirm results published previously by the inventor of this test (Nashed, 1981).
本报告对腹膜细胞致癌性试验进行了评估，该试验是由纳什（1981年）发明的一种新的短期体内-体外转化试验。实验设计和所用材料尽可能与纳什（1981年）所用的一致。受试化合物为两对致癌/非致癌类似物，即苯并[a]芘/芘和2-乙酰氨基芴/4-乙酰氨基芴。所有4种化合物均经口服给药。腹腔注射氢氧化铝作为腹膜巨噬细胞的促有丝分裂原。转化的标准是巨噬细胞在软琼脂中的集落生长。在所使用的条件下，两种转化细胞类型形成了集落。当添加集落刺激因子时，肺巨噬细胞和腹膜巨噬细胞形成了集落。在对256只受试动物（100只经致癌物处理、80只经非致癌类似物处理和76只未处理的对照）的巨噬细胞培养平板上未观察到集落生长。因此，我们无法证实该试验的发明者（纳什，1981年）先前发表的结果。
