Nomura Y, Okuma Y, Segawa T, Schmidt-Glenewinkel T, Giacobini E
Neurochem Res. 1981 Apr;6(4):391-400. doi: 10.1007/BF00963854.
The active uptake of [3H]pipecolic acid increased with incubation time and its uptake at 3 min was half of that at 20 min. On the other hand, a ratio (pellet/medium) of [3H]pipecolic acid uptake into glial cell-enriched fractions, was much less (0.4 - 0.6) than that of [14C]GABA (25.8 - 74.1). GABA, 10(-4) M, and pipecolic acid, 10(-4) M, produced a significant inhibitor of [3H]pipecolic acid uptake into P2 fractions. Pipecolic acid, 10(-4) M, significantly reduced the synaptosomal and glial uptake of [14C]GABA. GABA, 10(-4) M, affected neither spontaneous nor high K+-induced release of [3H]pipecolic acid from brain slices. It is suggested that pipecolic acid is involved in either synaptic transmission or in its modulation at GABA synapses in the central nervous system.
[3H]哌啶酸的主动摄取随孵育时间增加，其在3分钟时的摄取量是20分钟时的一半。另一方面，[3H]哌啶酸摄取到富含胶质细胞的组分中的比例（沉淀/培养基）比[14C]GABA的比例（25.8 - 74.1）低得多（0.4 - 0.6）。10^(-4) M的GABA和10^(-4) M的哌啶酸对[3H]哌啶酸摄取到P2组分中产生显著抑制作用。10^(-4) M的哌啶酸显著降低了[14C]GABA的突触体摄取和胶质摄取。10^(-4) M的GABA既不影响脑片自发释放也不影响高钾诱导释放的[3H]哌啶酸。提示哌啶酸参与中枢神经系统中GABA突触的突触传递或其调节。
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