Naftchi N E, Abrahams S J, Crain S M, Peterson E R, Hiller J M, Simon E J
Peptides. 1981;2 Suppl 1:57-60. doi: 10.1016/0196-9781(81)90056-5.
Spinal cord explants with attached dorsal root ganglia (DRGs), from 14-day fetal mice were fixed at 1-3 weeks in vitro and incubated for leucine-enkephalin (LE) immunoreactivity by the peroxidase-anti-peroxidase (PAP) immunohistochemical method. Results show long processes with labeled varicosities seen more often in dorsal regions of the cord explants. Stained punctate bodies and varicosities were often seen close to large cells in these cultures, whereas no label was detected in neuronal perikarya. A prominent laminar array of stained punctate bodies was noted in one cord explant, concentric with the perimeter of the explant. No LE label was detected in the neuritic outgrowths from the cord-DRG explants, whereas high levels of opiate receptors develop in these outgrowths, primarily on the DRG neurites, by 1-2 weeks in culture. The results indicate the presence of LE in explants of fetal mouse spinal cord with attached DRGs and offer an in vitro model system in which the onset and development of peptidergic neurons can be studied as they form functional cellular interrelationships with neurons bearing opioid and monoaminergic receptors in these organotypic cultures.
取14日龄胎鼠带有背根神经节（DRG）的脊髓外植体，在体外培养1 - 3周后固定，采用过氧化物酶 - 抗过氧化物酶（PAP）免疫组织化学方法进行亮氨酸脑啡肽（LE）免疫反应性孵育。结果显示，在脊髓外植体的背侧区域更常看到有标记膨体的长突起。在这些培养物中，常在大细胞附近看到染色的点状小体和膨体，而在神经元胞体中未检测到标记。在一个脊髓外植体中，观察到一层显著的染色点状小体排列，与外植体的周边同心。在脊髓 - DRG外植体的神经突起生长中未检测到LE标记，而在培养1 - 2周时，这些生长物中，主要是DRG神经突上，会形成高水平的阿片受体。结果表明，在带有DRG的胎鼠脊髓外植体中存在LE，并提供了一个体外模型系统，在这些器官型培养物中，肽能神经元在与带有阿片样和单胺能受体的神经元形成功能性细胞间关系时，其起始和发育过程可以得到研究。
