Akatov A K, Prokhorov V Ia, Witte W, Kühn M, Seltmann G
Zh Mikrobiol Epidemiol Immunobiol. 1978 Nov(11):47-52.
Experiments were conducted on 1500 albino mice; protective activity of 7 batches of S. aureus capsular antigen isolated from 5 strains was studied. The animals were immunized subcutaneously with different doses of the preparation (from 0.001 to 10000 microgram) once, twice, and thrice, and infected intraperitoneally with 6 encapsulated cultures. Capsular antigen proved to possess weak protective properties. The following regularities were revealed: 1) high antigen doses (over 5 microgram) increased mouse sensitivity to staphylococcus infection; 2) the greatest protective activity was shown by doses within 0.01-1 microgram; 3) there was no distinct "dose-effect" dependence; 4) single immunization was less effective than double and triple; 5) antigenic specificity of the capsular material was not connected with its protective properties.
对1500只白化小鼠进行了实验；研究了从5株菌株中分离出的7批金黄色葡萄球菌荚膜抗原的保护活性。用不同剂量（0.001至10000微克）的制剂对动物进行皮下免疫，分别免疫一次、两次和三次，然后腹腔注射6种荚膜培养物进行感染。结果证明荚膜抗原具有较弱的保护特性。揭示了以下规律：1）高剂量抗原（超过5微克）会增加小鼠对葡萄球菌感染的敏感性；2）0.01至1微克范围内的剂量显示出最大的保护活性；3）不存在明显的“剂量-效应”依赖性；4）单次免疫不如双次和三次免疫有效；5）荚膜物质的抗原特异性与其保护特性无关。
