蛋白质质量斜率测定中的曲率问题。
The problem of curvature in slope assays for protein quality.
作者信息
McLaughlan J M
出版信息
Adv Exp Med Biol. 1978;105:155-63. doi: 10.1007/978-1-4684-3366-1_10.
The dose-response curves in a valid slope--ratio assay must be linear and meet at the zero protein dose. However in rat bioassays at least 3 types of curvature are found when the method is applied to a wide range of samples; the types of curvature result from: 1) excessive protein 2) lysine deficiency and 3) threonine deficiency. Whole wheat flour which was limiting in lysine and threonine showed marked downward curvature after supplementation with threonine. It can be argued from these results that the slope ratio and relative protein value method underestimated the protein quality of lysine--deficient foods.
在有效的斜率比测定中，剂量-反应曲线必须是线性的，并且在零蛋白质剂量处相交。然而，在大鼠生物测定中，当该方法应用于广泛的样品时，至少会发现3种类型的曲线；这些曲线类型是由以下原因导致的：1）蛋白质过量；2）赖氨酸缺乏；3）苏氨酸缺乏。赖氨酸和苏氨酸含量有限的全麦面粉在补充苏氨酸后显示出明显的向下弯曲。从这些结果可以推断，斜率比和相对蛋白质值方法低估了赖氨酸缺乏食物的蛋白质质量。
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