Griffis L C, Henderson T R, Pickrell J A
Am Ind Hyg Assoc J. 1981 Aug;42(8):566-9. doi: 10.1080/15298668191420288.
Inhalation toxicology studies of glass fibers require a knowledge of the dose deposited in the lungs of exposed animals. A method is described which uses the quantity of acid-insoluble sodium present in rat lungs to determine the lung burdens of glass by mass after exposure to glass fiber aerosol. The glass fibers used contained 7.3% sodium. This sodium was measured in lung homogenates after acid washing and fusion in Li2CO3. The lower limit of detection was estimated as 40 microgram of this glass in rat lung. Results of an in vitro dissolution study indicated that a portion of the sodium present in the glass fibers was dissolved at a faster rate than the glass itself. This preferential dissolution may result in a small (5-25%) underestimation of glass in lung. This method was used to determine lung burdens of glass in rats after 1, 2, 4 or 5 exposures to a glass fiber aerosol (count median diameter: 0.11 micrometer, count median length: 8.3 micrometer). Five 6-hour exposures at an average concentration of 47 mg/m3 resulted in a lung burden of 176 microgram of glass. It was estimated that 41-48% of the lung burden was cleared between daily exposures.
玻璃纤维的吸入毒理学研究需要了解暴露动物肺部沉积的剂量。本文描述了一种方法，该方法利用大鼠肺中酸不溶性钠的含量来确定暴露于玻璃纤维气溶胶后肺中玻璃的质量负荷。所用的玻璃纤维含7.3%的钠。酸洗涤并在碳酸锂中熔融后，在肺匀浆中测量该钠。大鼠肺中该玻璃的检测下限估计为40微克。体外溶解研究结果表明，玻璃纤维中存在的一部分钠的溶解速度比玻璃本身更快。这种优先溶解可能导致肺中玻璃的含量被低估5-25%。该方法用于测定大鼠在1、2、4或5次暴露于玻璃纤维气溶胶（计数中值直径：0.11微米，计数中值长度：8.3微米）后肺中的玻璃负荷。在平均浓度为47毫克/立方米的情况下进行五次6小时暴露，导致肺中玻璃负荷为176微克。据估计，每日暴露期间，41-48%的肺负荷被清除。
