Blumfelder T, Logue G
Am J Hematol. 1981;11(1):77-84. doi: 10.1002/ajh.2830110109.
The amount of IgG in the serum of patients with suspected immune neutropenia that binds to normal paraformaldehyde-fixed human granulocytes was measured simultaneously by a quantitative antiglobulin consumption assay and by binding of 125I-staphylococcal protein A (SPA). There was a significant linear relationship between the results of these two assays for the sera of 42 different patients. However, SPA binding appeared more sensitive than the quantitative antiglobulin assay for determining IgG antigranulocyte antibodies in serum. In a patient with Felty's syndrome who underwent splenectomy, the results of both assays on sequential serum samples correlated with clinical improvement. Thus, SPA binding appears to be a sensitive and reliable technique for measuring antigranulocyte antibodies, and there is a close correlation between antibody measured by antiglobulin consumption and those detected by SPA binding.
通过定量抗球蛋白消耗试验和125I-葡萄球菌蛋白A（SPA）结合法，同时测定疑似免疫性中性粒细胞减少症患者血清中与正常多聚甲醛固定的人粒细胞结合的IgG量。对于42例不同患者的血清，这两种检测方法的结果之间存在显著的线性关系。然而，在测定血清中的IgG抗粒细胞抗体时，SPA结合法似乎比定量抗球蛋白试验更敏感。在一名接受脾切除术的费尔蒂综合征患者中，连续血清样本的两种检测结果均与临床改善情况相关。因此，SPA结合法似乎是一种测量抗粒细胞抗体的敏感且可靠的技术，通过抗球蛋白消耗法测得的抗体与通过SPA结合法检测到的抗体之间存在密切相关性。
