Kirby M L
Anat Rec. 1981 Jun;200(2):231-7. doi: 10.1002/ar.1092000211.
A morphometric analysis has been done on developing rat substantia gelatinosa of the lower cervical and upper thoracic levels of the spinal cord starting on the 15th day of gestation. The following parameters were measured: cell body diameter, cytoplasmic/nuclear areas, synaptic density, synaptic type and vesicle morphology of the presynaptic terminal in axodendritic synapses. Cell body size and cytoplasmic/nuclear areas of gelatinosal cells increase until the 15th day postnatally and then decrease somewhat to the adult values. The first synapses are seen on gestation day 17. Synaptic density increases linearly until the third day postnatally. Axodendritic synapses are most common throughout development and in the adult, while the proportion of axoaxonic synapses increases and axosomatic synapses decreases during development. Most of the terminals in axodendritic synapses contain clear-spherical vesicles but the occurrence of clear-flat vesicles and dense-cored vesicles in the terminals increases during development. It appears that these morphological parameters provide a stable index of development in the substantia gelatinosa which can be correlated with functional devleopment of the area. Hopefully, they will provide a means to asses subtle anomalies induced by nonteratogenic drugs or other environmental changes.
从妊娠第15天开始，对发育中的大鼠脊髓下颈段和上胸段的胶状质进行了形态计量学分析。测量了以下参数：细胞体直径、细胞质/细胞核面积、突触密度、突触类型以及轴突-树突突触中突触前终末的囊泡形态。胶状质细胞的细胞体大小和细胞质/细胞核面积在出生后第15天之前增加，然后略有下降至成年值。在妊娠第17天可见第一批突触。突触密度在出生后第三天之前呈线性增加。在整个发育过程以及成年期，轴突-树突突触最为常见，而轴突-轴突突触的比例在发育过程中增加，轴突-胞体突触减少。轴突-树突突触中的大多数终末含有清亮球形囊泡，但终末中清亮扁平囊泡和致密核心囊泡的出现率在发育过程中增加。看来这些形态学参数为胶状质的发育提供了一个稳定的指标，可与该区域的功能发育相关联。有望它们将提供一种手段来评估由非致畸药物或其他环境变化引起的细微异常。
