人淋巴细胞中腺苷脱氨酶的放射性同位素微量测定
Radioisotopic microassay of adenosine deaminase in human lymphocytes.
作者信息
Vanella A, Giustolisi R, Geremia E, Guglielmo P, Tiriolo P, Pinturo R, Di Silvestro I
出版信息
Biochem Exp Biol. 1980;16(3):247-50.
PMID:7272086
Abstract
A radioisotopic method for a rapid assay of adenosine deaminase in human lymphocytes is proposed here. [2-3H] adenosine, as substrate, has been employed for the enzymatic assay. The products of reaction have been resolved by thin layer chromatography on PEI cellulose. The plates were developed with distilled water and inosine spots absorbing in the U.V. were eluted with 0.1 N HCl. The eluates obtained from the inosine spots were employed for radioactive measurements.
摘要
本文提出一种用于快速测定人淋巴细胞中腺苷脱氨酶的放射性同位素方法。以[2-³H]腺苷作为底物用于酶促测定。反应产物通过在聚乙烯亚胺纤维素上进行薄层层析进行分离。薄板用蒸馏水展开，在紫外线下吸收的肌苷斑点用0.1N盐酸洗脱。从肌苷斑点获得的洗脱液用于放射性测量。
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