Emmanuel F X, Vaughan A T, Catty D
Br J Cancer. 1981 Aug;44(2):160-5. doi: 10.1038/bjc.1981.166.
Treatment of BALB/c mice with radioactive isotopes of the bone-seeking element strontium reduces the percentage of specific NK-cell cytotoxicity to only 2.6%, compared with 13.6% for normal BALB/c and 36.3% for athymic (nude) BALB/c. The syngeneic plasmacytoma NS-1 was used as target in a 4th in vitro NK-cell microassay. Marrow cellularity in treated mice is reduced to 12.5% of controls, but haemopoietic and stem-cell functions are taken over by the spleen and the peripheral blood picture remains relatively normal. Allogenic (H-2k) tumour transplants are rejected normally with good anti-H-2k alloantibody response. Haemopoietic and T- and B-cell functions are therefore substantially intact, and the defect seems confined to NK cells. In vivo, after s.c. inoculation of 10(6) NS-1 cells, 8/12 controls grew a solid tumour after a mean delay of 30.5 +/- 1.25 (s.e.) days, whereas 5/6 90Sr-treated mice grew the tumours after a delay of only 10.5 +/- 1.8 days. This markedly reduced delay in the 90Sr-treated mice lends support to suggestions that NK cells play an important role in resisting the establishment of tumour foci (i.e. in antitumour surveillance). Mice treated with 90Sr could be useful in evaluating the in vitro role of NK cells.
用亲骨性元素锶的放射性同位素处理BALB/c小鼠，可使特异性自然杀伤细胞（NK细胞）的细胞毒性百分比降至仅2.6%，而正常BALB/c小鼠为13.6%，无胸腺（裸）BALB/c小鼠为36.3%。在第四次体外NK细胞微量测定中，同基因浆细胞瘤NS-1被用作靶细胞。经处理的小鼠骨髓细胞数量降至对照组的12.5%，但其造血和干细胞功能被脾脏接管，外周血情况仍相对正常。同种异体（H-2k）肿瘤移植能够正常被排斥，伴有良好的抗H-2k同种异体抗体反应。因此，造血以及T细胞和B细胞功能基本完好，缺陷似乎仅限于NK细胞。在体内，皮下接种10(6)个NS-1细胞后，12只对照组小鼠中有8只在平均延迟30.5±1.25（标准误）天后长出实体瘤，而6只经90Sr处理的小鼠中有5只仅在延迟10.5±1.8天后就长出了肿瘤。90Sr处理小鼠的这种明显缩短的延迟时间支持了NK细胞在抵抗肿瘤病灶形成（即在抗肿瘤监测）中起重要作用的观点。用90Sr处理的小鼠可能有助于评估NK细胞在体外的作用。
